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KMID : 0350519930460041631
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Journal of Catholic Medical College
1993 Volume.46 No. 4 p.1631 ~ p.1639
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Mutations of the p53 Tumor Suppressor Gone in Human Epithelial Ovarian Cancer
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Abstract
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p53 gene on chromosome 17p is considered to be a tumor suppressor gene, and frequent mutations in highly conserved regions of the p53 gene have been found in a wide variety of human cancers. Mutations in this portion of the gene are known to lead
to
the loss of the oncosuppressive potential of p53.
To detect in a more sensitive manner p53 gene mutations in 28 human epithelial ovarian cancers we utilized the polymerase chain reaction-singel strand conformation polymorphism(PCR-SSCP) technique.
Using PCR primers for the regions of the p53 gene, including exons 4-9, p53 mutations were detected in 35.7%(10 of 28) of the ovarian cancers. Of the 10 mutations observed, one was found in exon 4, seven in the region encompassing exons 5 and 6,
one in
exon 7 and one in the region encompassing exons 8 and 9. Mutations were clustered in exons 5 and 6 in highly conserved regions of the p53 gene.
These results suggest that the mutations of the p53 gene may play an important role in the development of buman epithelial ovarian cancers.
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KEYWORD
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